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KMID : 0379520050210010057
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한국독성학회지
2005 Volume.21 No. 1 p.57 ~ p.62
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The Chromosomal Aberration Test of Wild Ginseng Culture Extract in Chinese Hamster Lung Cell
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Song Si-Whan
Yang Deok-Chun
Choung Se-Young
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Abstract
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To investigate the mutant induction of wild ginseng culture extract, we performed chromosomal aberration assay with chinese hamster lung cell in vitro. The test concentration of the extract was decided for the standard with the 50% suppression of cell propagation in the cell. The concentrations for the chromosome test were 1,250, 2,500 and 5,000 ㎍/ml with metabolic activation (+S, 6 hours treatment), 1,100, 2,200 and 4,400 ㎍/ml without metabolic activation (-S, 6 hours treatment) 800, 1,600 and 3,200 ㎍/ml without metabolic activation (-S, 24 hours treatment). No significant increase in chromosome aberrations was observed at any of these concentrations both in the absence and presence of metabolic activation system. Cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS) caused a significant increase in chromosome aberration. These results may be concluded that wild ginseng culture extract is not capable of inducing chromosome aberration in cultured chinese hamster lung cell regardless of metabolic activation and genotoxicity of that is negative under the present experimental condition.
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KEYWORD
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Wild ginseng culture extract, Chromosomal aberration assay, Cyclophosphamide monohydrate, Ethylmethanesulfonate
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